Presence and Bacterial Load of Coliform in Selected Rivers of Bacolod City

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Nicole Mae B Buslay
Jearim Leandro P Famoso
Krizza Louise S Jalandra
Kayzylyn Ann R Regalado
Ruby Ann V Untalan
Maraille Zoie T Yangco
Czarina Mae G Ferrer

Abstract

Coliform is a probable indicator of fecal contamination in a given water sample that may cause disease in humans. This quantitative-descriptive research focuses only on coliform species. The main objectives of this research are to identify the type of coliform being isolated, to ascertain the mean Most Probable Number (MPN) per 100 mL of coliform, to distinguish which river has the highest number of coliform, and to determine if there is a significant difference in the mean MPN per 100 mL among the three selected rivers. Water samples were obtained using sterile bottles, followed by proper aseptic technique. Using a cooler, water samples were transported to the laboratory and tested using the Multiple Tube Fermentation Technique to detect coliform species. Coliform Identification showed the presence of the coliform genera in the river samples: Escherichia, Klebsiella, Enterobacter, Salmonella, and Citrobacter. Rivers tested showed a mean MPN per 100 mL of 2.15 x 105, 1.25 x 105, and 1.05 x 105 for Alunan, Magsungay, and Sum-ag, respectively. Alunan River had the highest number of coliform isolated. The results showed non significant difference in the mean MPN per 100 mL. The researchers concluded that the three selected rivers were contaminated and unsafe. Thus, they could be risks to the health of the people in the vicinity.

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